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. 2016 Jul 1;30(13):1558–1572. doi: 10.1101/gad.280222.116

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© 2016 Lemay et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — Seb1 levels affect the cotranscriptional assembly of the cleavage/poly(A) machinery. (A) Bars above the rps2 gene show the positions of PCR products used for ChIP analyses. (B–E) ChIP assays of TAP-tagged versions of Rna14 (B), Clp1 (C), Ysh1 (D), and Cft2 (E) in wild-type and Seb1-depleted cells (P_nmt1-seb1). An untagged control strain was used to monitor the background signal of the ChIP assays. (F–I) Recruitment of 3′ end processing factors as a ratio of total RNAPII at the 3′ end of rps2 (region 2). ChIP signal of TAP-tagged 3′ end processing factors was divided by the total RNAPII signal at region 2. Region 2 was analyzed because it represents the location of maximal 3′ end processing factor recruitment. Error bars indicate SD. n = 3 biological replicates from independent cell cultures. (*) P < 0.05, Student's t-test.