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. Author manuscript; available in PMC: 2016 Jul 19.
Published in final edited form as: Cell Metab. 2010 Oct 6;12(4):329–340. doi: 10.1016/j.cmet.2010.08.015

Figure 1. In vivo podocyte specific IR knockdown in transgenic mice.

Figure 1

Figure 1

A. Glomeruli of Z/EG reporter mice immuno-stained with an anti GFP antibody. Specific excision demonstrated in podocytes (arrowed).

B. Glomeruli were extracted and purified using dynabeads. This is light microscopic analysis of the extract showing a pure glomerular population and the beads.

C. PCR of genomic DNA from different tissues in podIRKO compared with (cw) IRfl/fl cre negative mice. Only podIRKO glomeruli have a smaller excised genomic DNA band present [arrowed] (G=glomeruli S=spleen E= ear).

D. Podocyte isolation of CFP positive podocytes from 3 week old podIRKO and control mice demonstrate that the IR mRNA is knocked down by over 90% in podocytes of the podIRKO mice compared with other glomerular cells (OGC) which were CFP negative. In control mice the IR is increased by 60% in the podocyte fraction.

E. There is increased production (100%) of IGF-1 receptor mRNA in podocyte fraction of podIRKO mice compared with a 50% reduction in the control mice.