Table 3.
Phototoxic effect of PPArg2, ZnPC and RB on clinical S. aureus strains.
| Strain | Mean reduction of survivala (log10 CFU) ± SD | ||
|---|---|---|---|
| PPArg2b | ZnPCb | RBc | |
| 1764/p | 5.53 ± 0.01 | 3.49 ± 0.77 | 5.47 ± 0.67 |
| 6452 | 2.05 ± 0.72 | 3.76 ± 0.45 | 3.60 ± 1.32 |
| 6987 | 5.29 ± 0.01 | 2.89 ± 0.55 | 3.37 ± 0.65 |
| 473 | 5.45 ± 0.04 | 4.90 ± 0.18 | 5.42 ± 0.93 |
| 469 | 3.53 ± 0.55 | 5.77 ± 0.10 | 1.69 ± 0.12 |
| 146 | 4.46 ± 0.30 | 4.56 ± 1.06 | 4.48 ± 1.30 |
| 5491 | 0.61 ± 0.20 | 2.94 ± 0.64 | 2.89 ± 0.31 |
The values were calculated by subtracting log10 CFU/ml of treated samples from those of untreated controls (0 J/cm2; 0 μM PS). At least three biological replicates were used for the calculation of the mean reduction values. SD–Standard Deviation. Underlined values indicate statically significant reduction relative to light control (20 J/cm2; 0 μM PS) (p < 0.05). Bold values indicate a bactericidal effect (>3 log10 reduction units).
Photosensitizer excited with the red light (maximal intensity at λmax 627 nm). Concentrations used: PPArg2 20 μM; ZnPC 5 nM. Light dose applied was 20 J/cm2.
0.1 μM RB excited with the green light (maximal intensity at λmax 520 nm). Light dose applied was 20 J/cm2.
PPArg2–Protoporphyrin IX diarginate; ZnPC–Zinc Phthalocyanine; RB–rose Bengal.