Fig. 2.

Development of the tissue-engineered construct. (a) Immunohistochemical analysis of the basic TEC stained with type I collagen (Col I), type II collagen (Col II), type III collagen (Col III), fibronectin, and negative IgG (control). Bar = 100 mm. (b) Macroscopic view of the TEC, which was integrated to one spherical body. The diameter of this TEC was 5 mm and the thickness was 2 mm. (c) Microscopic view of HE staining (left side) and fibronectin staining (right side) of the cultured porcine chondral fragment for 7 days after the implantation of the basic TEC on the injured surface. Bar = 100 mm.

Cited from Ref. 69.