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. 2016 Jul 19;6:29889. doi: 10.1038/srep29889

Figure 2. Gp96 activated NK cells do not directly lyse tumor cells but are necessary for tumor- specific CTL function.

Figure 2

(A–F) Mice were immunized twice, one week apart with 2 μg of D122 or non-tumor derived gp96 and sacrificed 2 weeks later. (B) T cells were isolated from the spleens of immunized mice and, (C) incubated with labeled D122 target cells in a CTL assay. (D) NK cells were isolated from spleens of immunized mice and incubated with (E) D122 target cells or (F) YAC cells and killing was measured. (G) Immunized mice were treated with anti-NK1.1 or mIgG prior to challenge with D122 tumor cells. Three days following challenge with D122, mice were sacrificed and T cells were isolated from draining lymph nodes. (H) Cytotoxicity of isolated T cells were assayed using D122 target cells. (I) T cells in draining lymph nodes from immunized and challenged mice were counted. Statistical analysis was performed by ANOVA followed by Bonferroni post-test *p < 0.05, **p < 0.01, ***p < 0.001.