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. 2016 Jul 19;6:29878. doi: 10.1038/srep29878

Figure 3. SNRPN downregulated the expression of Nr4a1.

Figure 3

(a) E15 cortical neurons were electroporated with control (pCAG-IRES-GFP) vector or HA-SNRPN plasmid. 72 h later, Nr4a1 mRNA was quantified by qPCR. (b) Western blot showed the change in the Nr4a1 protein level by SNRPN overexpression in cultured cortical neurons. Anti-HA antibody was used to detect the effect of HA-tagged SNRPN plasmid. Anti-SNRPN antibody was used to examine exogenous and endogenous SNRPN proteins, including SmN and SmB. The band about 30 kDa represents exogenous HA-tagged SmN. (c) Quantification of the expression of exogenous SmN, endogenous SmN, SmB, and Nr4a1 in cultured cortical neurons transfected with control and HA-SNRPN plasmids. (d) Regulation of Nr4a1 protein level by SNRPN shRNA and control constructs (Scramble) in cultured cortical neurons. SmN antibody was used to detect the effect of SNRPN shRNA. (e) Quantification of the expression of SmN, SmB, and Nr4a1 in cultured cortical neurons transfected with scramble and SNRPN RNAi plasmids. Data were normalized to GAPDH. The RNA or protein level of the control was set as ‘1’. Error bars, SEM. *P < 0.05, **P < 0.01 (Student’s t-test). Each experiment was repeated independently for at least 3 times.