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. 2016 Jul 19;6:29878. doi: 10.1038/srep29878

Figure 5. Nr4a1 knockdown rescued the effect of SNRPN RNAi.

Figure 5

(a) Images showed the cultured cortical neurons transfected with scramble, SNRPN RNAi, Nr4a1 RNAi, and SNRPN RNAi + Nr4a1 RNAi plasmids for 72 h. Cells were stained for GFP (green), DAPI (blue) and SMI-312 (red). Scale bar, 50 μm. (b,c) Average length of total neurites and axons of GFP + neurons. Numbers of neurons analyzed in each group: 18 for scramble group, 18 for SNRPN RNAi group, 18 for Nr4a1 group, 40 for SNRPN RNAi + Nr4a1 RNAi group. (d) Embryonic cortical neurons transfected with SNRPN RNAi and scramble plasmids were treated by DIM and DMSO for 48 h in vitro, respectively. Representative images show cells stained for GFP (green), DAPI (blue) and SMI-312 (red). Scale bar, 50 μm. (e,f) Average length of total neurites and axons of GFP+ neurons. Numbers of neurons analyzed in each group: 22 for scramble + DMSO group, 33 for SNRPN RNAi + DMSO group, 8 for scramble + DIM group, 15 for SNRPN RNAi + DIM group; Error bars, SEM. *P < 0.05, **P < 0.01 (Student’s t-test).