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. 2016 Jul 19;6:29868. doi: 10.1038/srep29868

Figure 3. Analysis of 13 selected compounds in keratinocytes.

Figure 3

(A–C) Protein expression analysis in HaCaT total cell lysates stimulated (+) or not (−) with 5 ng/ml TGF-β for 96 h. In (A) cells were co-treated with DMSO or specific EPMs (10 μM). In (B) cells were co-treated with DMSO (0 μM) or EPM-1 and EPM-13 at the indicated concentrations. In (C) cells were co-treated with DMSO (0 μM) or EPM-1 and EPM-13 (10 μM) at the indicated time points following TGF-β stimulation. Immunoblots for the indicated proteins and Gapdh, the protein loading control, are shown. (D) E-cadherin immunofluorescence microscopy of HaCaT cells stimulated with vehicle or 5 ng/ml TGF-β for 96 h in the presence of DMSO or 10 μM of EPM-13 or 3.3 μM GW6604 (GW). (E) Actin microfilament direct fluorescence microscopy of HaCaT cells treated as in panel D. White bars indicate 10 μm.