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. 2016 Jul 19;6:29898. doi: 10.1038/srep29898

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Copyright © 2016, Macmillan Publishers Limited

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Effect diabetes induced by a single injection of alloxan in fed and fasted (48 h) animals on plasma glucose (A) insulin (B) and irisin (C) levels and FNDC5 gene expression levels in the hypothalamus (D), muscle (E) and epididymal (F) WAT of adult male rats. *P < 0.05 vs. fed animals. ^#P < 0.05 vs. saline. (Two-way ANOVA with post hoc Tukey’s test, a value of P < 0.05 was considered statistically significant). Values are expressed in mg/dl for plasma glucose levels, ng/ml for plasma insulin levels and μg/ml for circulating irisin levels. Arbitrary units were used for FNDC5 gene expression, as the mean ± SEM, where rats fed and injected with saline = 100%. N = 8 (saline treatment) or 10 (alloxan treatment).