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. 2016 Jul 19;6:29880. doi: 10.1038/srep29880

Figure 4. Role of the NOS2-NO-COX2 pathway in endothelial cell-osteoblast crosstalk.

Figure 4

Mouse primary osteoblasts were treated with 1g- and 0.008g-EC-CM. Real time RT-PCR of (a) Nos2 and (b) Cox2 normalized versus Gapdh. Mouse primary osteoblasts were treated with 1g- or 0.008g-EC-CM in the absence or in the presence of the NOS2 inhibitor 1400 W. (c) RT-PCR of Cox2 and Cyclin D1 normalized versus Gapdh. (d) MTT proliferation assay. (e) Representative images of ALP cytochemical staining. (f) Densitometric quantification of ALP staining. Osteoblasts were treated with 1g- and 0.008g-EC-CM in the absence or presence of the COX inhibitor acetylsalicylic acid. (g) RT-PCR analysis of Cyclin D1 and Alp normalized versus Gapdh. Images are representative and data are the mean ± SD of at least 3 independent experiments (Student’s t test). Bar = 100 μm. All gels have been run under the same experimental conditions.