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. 2016 Jul 19;6:29789. doi: 10.1038/srep29789

Figure 7. VCE-003.2 is neuroprotective in 3NP-intoxicated mice.

Figure 7

(a) Behavioural score was determined 12 hours after 3NP intoxication. Mice were treated with VCE-003.2 (20 mg/Kg) alone or combined with T0070907 (5 mg/kg) as indicated. Animals were also treated separately with either VCE-003.2 or T0070907 alone. Hind limb clasping, general locomotor activity, hind limb dystonia and kyphosis were rated from 0 to 2 based on severity (score 0 indicates normal function and 2 indicates the most severe affected function). Values are expressed as means ± SEM (n = 6). (b) Representative images of Nissl staining performed on coronal striatal brain sections from the same mice groups. Photomicrographs of NeuN (Bar size, 50 μm) Iba-1 and glial fibrillary acidic protein (GFAP) immunostained sections through the different group of mice are also shown. (c) Quantification of the different markers was performed with Image J software. Total average number of neurons (NeuN+), microglia (Iba1+) and astrocytes (GFAP+) is shown. Values are expressed as means ± SEM (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001 vs. Vehicle; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. 3NP; †p < 0.05, ††p < 0.01, †††p < 0.001 vs. the 3NP + VCE-003.2 group.