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. 2016 Jul 18;214(2):167–179. doi: 10.1083/jcb.201511102

Figure 7.

Figure 7.

Binding of Myo51 tail constructs to actin or actin-Tpm. (A) 4–20% SDS gel showing FLAG affinity purified N-FLAG-Biotin Myo51 tail (amino acids 893–1,471 of full-length Myo51 heavy chain) constructs. Lane 1, molecular mass standards. Lane 2, Myo51 tail. Lane 3, Myo51 tail coexpressed with Rng8 and Rng9. (B) Actin or actin-Tpm filaments bound to Myo51 tail or Myo51 tail-Rng8/9 coated surfaces. Bar, 15 µm. (C) 12% SDS gel showing the supernatant (S) and the pellet (P) fractions after high-speed centrifugation of Myo51 tail or Myo51 tail–Rng8/9 incubated with or without actin/actin-Tpm. M, molecular mass standards. The majority of Myo51 tail-Rng8/9 cosedimented with actin-Tpm, but not with bare actin. Myo51 tail mostly stayed unbound to actin or actin-Tpm.