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. 2016 Jul 18;214(2):155–166. doi: 10.1083/jcb.201604081

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© 2016 Meitinger et al.

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Figure 1. — Genome-wide CRISPR/Cas9 screen for genes involved in activating p53 upon centrosome loss. (A, top) Immunofluorescence images of RPE1 cells, stained for DNA (red) and the centrosomal protein Cep192 (green), after treatment with DMSO or centrinone for 5 d. Bar, 10 µm. (bottom) Schematic highlighting the two classes of genes that would be identified in a centrinone-resistance screen. (B) Summary of the screen designed to identify genes that activate p53 in response to centrosome loss. (C) Table summarizing the results of two independent screens. All 15 colonies had one of the three listed genes deleted; no colony had more than one. (D) Schematic of the three proteins 53BP1, USP28, and TRIM37, identified by the screen.