Table 2. Nomenclature of cell types described in this study.
|
In vitro-generated APC: Harfuddin et al.14 | ||
|---|---|---|
| Type of APC | Classification | |
| 1 | Monocytes | Blood CD14+ monocytes |
| 2 | Macrophages | M-CSF generated |
| 3 | Immature conventional DC | GM-CSF, IL-4 generated |
| 4 | Mature conventional DC | GM-CSF, IL-4, + LPS, IFN-γ generated |
| 5 | Fc-monocytes | Fc protein generated |
| 6 | CD137L-DC | CD137-Fc protein generated |
| In vivo-derived APC: Segura et al.35 | ||
| Type of APC [vivo: in vivo] | Classification | |
| 1 | CD14 monocytevivo | CD14+CD16− monocytes |
| 2 | CD16 monocytevivo | CD14dimCD16+ monocytes |
| 3 | BDCA1+ DCvivo | HLA-DR+CD11c+CD14−CD16−BDCA1+ cells from blood |
| 4 | Inflammatory DCvivo | HLA-DR+CD11c+CD16−BDCA1+FcεRI+ cells from inflammatory fluids/ascites |
| 5 | Inflammatory macrophagevivo | HLA-DR+CD11c+CD16+BDCA1−FcεRI− cells from inflammatory fluids/ascites |
| Gene set from public domain: Derived by Segura et al.35 | ||
| Type of APC [PD: public domain] | Classification | |
| 1 | CD14 monocytePD | Blood CD14+ monocytes |
| 2 | CD16 monocytePD | Blood CD16+ monocytes |
| 3 | Monocyte-derived DCPD | In vitro generated monocyte-derived DC |
| 4 | MacrophagePD | PBMC derived, monocyte derived, and alveolar macrophages |
| 5 | BDCA1 DCPD | Blood and tonsil BDCA1+ DC |
The BDCA1+ DC gene signature is derived from an in vivo DC subset (BDCA1+ DC), while the macrophage gene signature is a combination derived from in vitro generated macrophage gene signatures and in vivo alveolar macrophage gene signature3534. The moDC gene signature is derived from in vitro monocyte-derived DCs that were generated by GM-CSF and IL-4. The CD14 and CD16 gene signatures are from blood CD14+ and CD16+ monocytes, respectively. Segura and colleagues acquired the transcriptome of in vivo inflammatory DCs and inflammatory macrophages that were isolated from the synovial fluid of rheumatoid arthritis patients and inflammatory tumour ascites, along with other in vivo APCs. Table 2 describes the cell populations used in this study. To unequivocally distinguish the cell types we add the suffix ‘vivo’ to in vivo APCs, while ‘PD’ refers to APC gene sets acquired from public databases by Segura et al.35.