Table 1.
Primary antibodies, sources, dilutions and pre‐treatments
| Primary Antibody | Clone | Dilution | Pre‐treatment | Source |
|---|---|---|---|---|
| Alkaline phosphatase | Poly rabbit | 1:100 | Boil in 0.1 m citrate buffer 20 min (steamer) | Gene Tex, Irvine, CA, USA |
| Ezrin | Mono mouse, Clone 18 | 1:300 | No pre‐treatment | BD Transduction Laboratories, San Jose, CA, USA |
| Metastasin (S100A4) | Poly rabbit | 1:150 | Boil in citrate buffer 20 min (steamer) | Thermo Scientific, Fremont, CA, USA |
| Ki‐67 | Mono mouse, Clone 7B11 | 1:100 | Boil in citrate buffer 20 min (steamer) | Invitrogen, Camarillo, CA, USA |
| Collagen I | Poly goat | 1:30 | Digestion with 0.1% Pepsin/0.5 m acetic acid (Sigma) for 2 h (37°C) | Southern Biotech, Birmingham, AL, USA |
| Cytokeratin | Mouse mono, Clone AE1 + AE3 | 1:250 | Boil in Tris–EDTA (Dako) 20 min (steamer) | Cell Marque, Rocklin, CA, USA |
| Vimentin | Mouse mono, Clone V9 | 1:200 | Boil in citrate buffer 20 min (steamer) | Dako, Glostrup, Denmark |
| Osteocalcin | Poly rabbit | 1:400 | No pre‐treatment | AbD Serotec, Oxford, UK (Bio‐Rad Company) |