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letter
. 2016 Feb 3;590(3):349–357. doi: 10.1002/1873-3468.12065

Figure 5.

Figure 5

Blue native PAGE analysis of EsaA‐ and EssB‐containing complexes. Membrane fractions were prepared from strain RN6390 (WT) or the indicated deletion mutant, and samples (5 μg protein) were solubilised using 2% Fos‐choline‐12, as described under Experimental Procedures. The solubilised material was loaded onto a BN gel containing a gradient of 4–16% acrylamide. Subsequently, the proteins were transferred to PVDF membrane followed by immunological detection of A. EsaA or B. EssB. The arrows to the right indicate complexes containing EsaA (panel A) or EssB (panel B).