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. 2015 Feb 10;32(3):355–365. doi: 10.1002/yea.3063

Figure 4.

Figure 4

Inducible M–Track system. (A) Atg13–protA–H3 Atg1–Cnb1 or Atg13–protA–H3 yeast cells containing FKBP–HKMT or Atg1–HKMT on a centromeric plasmid were grown to mid‐log phase and treated with FK506 for 1 h. FK506 tethers HKMT to Cnb1. Protein isolation, TEV cleavage and western blotting was performed as in Figure 1C. Input extracts used for immunoprecipitation are also shown. (B) Atg13–protA–H3 Atg1–Cnb1 or wild‐type yeast cells containing FKBP–HKMT or an empty plasmid (pRS315) were grown to mid‐log phase, treated with FK506 for the indicated times, TCA precipitated and analysed by western blotting. FK506 was washed out by transferring the cells to fresh medium for the indicated times.