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. 2015 Feb 11;96(3):448–462. doi: 10.1111/mmi.12940

Figure 6.

figure

Use of anti‐GFP pull‐downs to isolate a thylakoid membrane fraction from low‐light S ynechocystis  ftsH2–gfp cells.

A. Silver‐stained SDS–PAGE gel for membrane fractions from wild‐type and ftsH2–gfp. M: crude membrane preparation (material containing 0.1 nmol chlorophyll loaded per lane); FT: flow‐through (i.e. unbound material) from the column; W1, W2: first and second column washes; E: final elute of bound material. Each of lanes FT, W1, W2 and E contains the appropriate fraction from material containing 0.2 nmol chlorophyll loaded onto the column.

B–E. Immunoblots with antibodies against GFP (B), FtsH2 (C), FtsH (global) (D) and D1 protein of PSII (E).