Figure 3.
Comparison of in vitro light-driven enzyme activity from N. benthamiana thylakoids harboring CYP79A1 enzyme variants. Assays were carried out in the presence or absence of 8.3 μM soluble Fd (+Fd or – Fd) as an electron carrier. Bars show average activities of four separate thylakoid preparations per construct, normalized to their relative protein levels as determined from immunoblotting experiments. Error bars indicate ± SD of biological replicates.