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. 2016 Apr 27;11(7):1862–1869. doi: 10.1021/acschembio.6b00190

Figure 4.

Figure 4

Effects of soluble ferredoxin and detergent on in vitro enzyme activity of C and CF proteins. (a) Light-dependent enzyme activity of thylakoids harboring either C or CF, driven by electron transfer from soluble or fused ferredoxin reduced by photosystem I. (b) Enzyme activity of dark-incubated thylakoids harboring C or CF, driven by electron transfer from soluble or fused ferredoxin reduced by 0.6 μM FNR in the presence of 0.5 mM NADPH. (c) Light-dependent activity of intact (-βDM) or solubilized (+βDM) thylakoids harboring C or CF in the presence of 8.3 μM soluble Fd. Experimental values are means of four technical repeats each, normalized to relative protein levels determined by immunoblot, with error bars showing ± SD. βDM, n-dodecyl β-D-maltoside.