Figure 5.

Effect of ionic strength on light-driven enzyme activity of tobacco thylakoids containing C (+soluble Fd) or CF (±soluble Fd). (a) Dependence of C and CF activity on ionic strength, adjusted with NaCl. (b) Dependence on ionic strength of C and CF activity measured in the presence of 0.6 μM FNR and 1.63 mM NADP⁺ acting as a competing electron sink for reduced Fd. Data are plotted relative to the maximum activity of C obtained in a. Curves show mean activities from (a) four or (b) two technical replicates, normalized to the relative levels of C and CF proteins. Error bars indicate ± SD.