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. Author manuscript; available in PMC: 2016 Jul 19.
Published in final edited form as: Cancer Res. 2011 Aug 18;71(20):6535–6546. doi: 10.1158/0008-5472.CAN-11-0329

Figure 5. Kiss1/Kiss1r regulates tumorigenesis by activating RhoA.

Figure 5

(A) Activation of Rho family of GTPases by suppressing Kiss1r expression. Kiss1r activates RhoA in a dose-dependent manner while Rac1 and Cdc42 are not affected by the downregulation of Kiss1r. (B) RhoA and its mutants regulate anchorage-independent colony formation in tumor cells isolated from PyMT/Kiss1r+/+ and PyMT/Kiss1r+/− mouse models. (C) Knockdown of KISS1R in Ras-transformed MCF10A cell inhibited anchorage-independent cell growth and colony formation. (D) Ras-induced anchorage-independent colony formation of MCF10A cells depends on RhoA signaling pathway using different RhoA plasmids or treated with RhoA inhibitor, Y-27632 (10μmol/L).