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. Author manuscript; available in PMC: 2016 Jul 19.
Published in final edited form as: J Neurochem. 2010 May 13;114(3):820–831. doi: 10.1111/j.1471-4159.2010.06807.x

TABLE 1.

Metabolites labeled via the TCA cycle in extracts of brain from 21–22 day old rats after injection of [2-13C]ALCAR

nmol 13C incorporated/mg protein#
15 min 60 min 120 min
[4-13C]Glutamate 0.378 ± 0.033** 0.605 ± 0.056 1.045 ± 0.158
[3-13C]Glutamate 0.544 ± 0.042 0.665 ± 0.105 1.010 ± 0.141
[2-13C]Glutamate 0.389 ± 0.067** 0.513 ± 0.075* 0.912 ± 0.171
[4-13C]Glutamine 0.180 ± 0.021** 0.212 ± 0.053** 0.477 ± 0.080
[3-13C]Glutamine 0.278 ± 0.022 0.305 ± 0.070 0.685 ± 0.091
[2-13C]Glutamine 0.263 ± 0.035 0.535 ± 0.168 0.471 ± 0.090
[3-13C]Aspartate 0.222 ±0.059 0.288± 0.142 0.486 ±0.104
[2-13C]Aspartate 0.042 ±0.009** 0.076± 0.007 0.134 ±0.029
[2-13C]GABA 0.110 ± 0.021 0.177 ± 0.085 0.141 ± 0.034
[3-13C]GABA 0.222 ± 0.053 0.231 ± 0.065 0.371 ± 0.083
[4-13C]GABA 0.127 ± 0.025*** 0.230 ± 0.051*** 0.582 ± 0.093
#

Data were obtained from peak integration of the 13C-NMR spectra as described in Methods. Percent enrichment was calculated from 13C labeling and total concentration as described in methods. Values are mean ± S.E. of data from n = 5 brains at 15 min, n=6 at 60 min, and n = 8 at 120 min. Data were analyzed using one-way ANOVA. Asterisks indicate values that are significantly different from 120 min time point,

*

p<0.05,

**

p<0.01

***

p<0.001.