TABLE 1.
nmol 13C incorporated/mg protein# | |||
---|---|---|---|
15 min | 60 min | 120 min | |
[4-13C]Glutamate | 0.378 ± 0.033** | 0.605 ± 0.056 | 1.045 ± 0.158 |
[3-13C]Glutamate | 0.544 ± 0.042 | 0.665 ± 0.105 | 1.010 ± 0.141 |
[2-13C]Glutamate | 0.389 ± 0.067** | 0.513 ± 0.075* | 0.912 ± 0.171 |
[4-13C]Glutamine | 0.180 ± 0.021** | 0.212 ± 0.053** | 0.477 ± 0.080 |
[3-13C]Glutamine | 0.278 ± 0.022 | 0.305 ± 0.070 | 0.685 ± 0.091 |
[2-13C]Glutamine | 0.263 ± 0.035 | 0.535 ± 0.168 | 0.471 ± 0.090 |
[3-13C]Aspartate | 0.222 ±0.059 | 0.288± 0.142 | 0.486 ±0.104 |
[2-13C]Aspartate | 0.042 ±0.009** | 0.076± 0.007 | 0.134 ±0.029 |
[2-13C]GABA | 0.110 ± 0.021 | 0.177 ± 0.085 | 0.141 ± 0.034 |
[3-13C]GABA | 0.222 ± 0.053 | 0.231 ± 0.065 | 0.371 ± 0.083 |
[4-13C]GABA | 0.127 ± 0.025*** | 0.230 ± 0.051*** | 0.582 ± 0.093 |
Data were obtained from peak integration of the 13C-NMR spectra as described in Methods. Percent enrichment was calculated from 13C labeling and total concentration as described in methods. Values are mean ± S.E. of data from n = 5 brains at 15 min, n=6 at 60 min, and n = 8 at 120 min. Data were analyzed using one-way ANOVA. Asterisks indicate values that are significantly different from 120 min time point,
p<0.05,
p<0.01
p<0.001.