(A to C) Summary of daily physical activity, including mean activity during 2-week period after gene transfer (A), duration of burst activity [>2000 arbitrary units (AU)] (B), and post-burst activity (C). (D) Representative 24-hour activity pattern in GFP- and TBX18-transduced animals. (E) Mean HR during maximal activity. (F) HRV (LF/HF) was associated with the duration of bursts of activity in a linear regression model. Each point corresponds to the duration of burst of activity (y axis) and LF/HF ratio (x axis). Data in (A) to (C) and (E) are means ± SEM (GFP, n = 5; TBX18, n = 7), and P values were determined by two-tailed t test. In (F), P value was determined by the linear regression model.