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. 2015 Jan 16;24(2):453–465. doi: 10.1111/mec.13041

Figure 1.

Figure 1

Colour polymorphism of Acropora millepora. (a) Different red fluorescent protein (RFP) levels accumulated by A. millepora colour morphs in response to identical environmental conditions in Florence Bay, Magnetic Island, Great Barrier Reef, Australia. (b) Representative images show the increased RFP content in the light‐exposed branch side (left) compared to the shaded branch side (right). (c) Lower panel: Fluorescence micrographs of three colour morphs (LR, low red; MR, medium red; HR, high red) cultured in the laboratory under the same light intensity showing different RFP (amilFP597) contents in the upper (Light) and lower (Shade) branch sides. Upper panel: Semi‐quantitative RT‐PCR analyses of RFP expression in the respective branch sides of LR, MR and HR morphs. Ethidium bromide‐stained agarose gels show the RFP and actin amplicons. (d) Mean RFP transcript levels normalized to the actin control in shaded (S) and light‐exposed (L) branch sides. Error bars denote standard deviation. Asterisks indicate significant differences (anova; P < 0.01) among samples from shaded and light‐exposed branch sides, respectively.