Figure 2.

Schematic representation of the amilFP597 gene within a gene tandem array. (a) The five exons of the gene are displayed as black bars with their length in nucleotides indicated beneath brackets. The grey‐shaded regions in exons 1 and 5 correspond to the 5′ and 3′UTRs, respectively. The chromophore‐forming amino acid triplet (DYG) is located within exon 3 and is marked by a white vertical line. The four introns are shown as white bars with their respective sizes. The analysed promoter region of the gene extends 1105 bp upstream of the 5′UTR. The positions of three indels (I1–I3) differentiating the indel (+) and the indel (−) promoter variants of amilFP597 are indicated in the promoter region. The DNA fragment linking a putative first amilFP597 copy with the analysed promoter region of the downstream copy is depicted by a dashed line. (b) Schematic representation of the analysed amilFP597 promoter region. The putative transcription initiation site (arrowhead) and the start codon (asterisk) are marked. The positions of the indel sequences (I1–I3) are marked by dotted lines, and their sequences are shown in boxes below the promoter diagram. The HpaI site in indel 2 used for restriction analysis is underlined.