Figure 4.

Missense mutations R380W and E215K are trafficked to the lysosome. Representative confocal microscopy images of HeLa cells transiently transfected with Krabbe mutations R380W (A) and E215K (B). Transfected cells were plated onto glass coverslips, fixed and immunostained for GALC (green), the lysosomal marker cathepsin D (red) or the ER marker calreticulin (red). Scale bar: 10 µm. C) Pearson's correlation coefficients were calculated for each of the mutations with both cathepsin D and calreticulin. Mean ± SEM for at least 20 individual cells from ≥3 independent experiments are shown. **p ≤ 0.005, *p ≤ 0.05, ns = not significant, calculated using a two‐tailed unpaired t‐test.