Skip to main content
. 2015 Apr 23;13(6):1113–1127. doi: 10.1111/jth.12884

Figure 2.

Figure 2

Iron deficiency (ID) increases ploidy in HEL. HEL and CMK cultured for 4 days in iron‐replete conditions (IR), ID, and IR plus 50 nmol L–1 PMA. (A) Representative images (×40) of nuclear staining (DAPI) of HEL and CMK after culture for 4 days in IR, ID, and IR media with 50 nmol L–1 PMA. Arrowheads depict large nuclei. (B) Flow cytometric measurement of Hoechst 33342 nuclear staining of HEL and CMK. Graphs depict the percentage of cells with ploidy greater than 4n as a fold change to IR. (C) Flow cytometric assessment of mean HEL and CMK cell volume depicted as fold change to IR. (D) Representative histograms of ploidy in HEL and CMK after 4 days’ culture in IR, ID, and IR + 50 nmol L–1 PMA. The results from two or three independent experiments are shown. **P ≤ 0.01, ***P ≤ 0.001.