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. 2015 Apr 23;13(6):1113–1127. doi: 10.1111/jth.12884

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© 2015 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals, Inc. on behalf of International Society on Thrombosis and Haemostasis.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Iron deficiency (ID) alters gene expression. Real‐time quantitative PCR analysis of custom targets and transcription factors in HEL, CMK, and megakaryocytes (MEGs) after 1, 2, and 5 days of culture, respectively. (A) Iron‐replete (IR) ΔC _t plotted against the ID ΔC _t of HEL, CMK, and MEGs after analysis of transcription factors and custom targets gene expression. Double lines represent thresholds of 0.5 and 2.0. (B) Relative mRNA quantity of genes that reached the set cutoffs of 0.5‐ and 2.0‐fold changes in at least one cell type under ID. Genes that were not detected are marked with x. Data were obtained from samples pooled from two or three independent experiments. FOV indicates field of view.