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. 2015 Apr 23;13(6):1113–1127. doi: 10.1111/jth.12884

Figure 5.

Figure 5

Regulation of genes in the hypoxia‐inducible factor (HIF) and vascular endothelial growth factor (VEGF) pathways is retained in subsequent experiments with iron deficiency (ID). Verification of selected identified regulated genes via real time quantitative PCR (RT‐PCR) in human erythroleukemia (HEL) (A) and megakaryoblastic leukemia (CMK) (B) after 3 days and megakaryocytes (MEGs) (C) and CD61‐negative cells (D) after 5 days of ID and iron‐replete condition (IR). Graphs depict the relative mRNA quantity (RQ) of selected genes in cells cultured under ID as compared to IR. All results are normalized to HPRT1.