Fig. 6. Immunoreactive nerve fibers in the ischemic (C and D) and sham control (A and B) bladder tissues are shown at ×400 magnifications. Progressive decrease in neural density was evident with increasing duration of ischemia. The number of immunopositive nerves decreased after 8-week bladder ischemia but did not reach significance. After 16-week bladder ischemia, immunoreactive nerve fibers density significantly decreased versus sham control. Arrows point to immunopositive nerve fibers. SE, standard error.