Table 1.
Four constructs were tested for fluorescent reporter expression during the differentiation of 3T3-L1 preadipocytes
| aADNp::promoted reporter protein | Day 4 | Day 5 | Day 6 | Day 7 | Day 10 | Day 14 |
|---|---|---|---|---|---|---|
| mRFP1 | + | ++ | +++ | +++ | +++ | +++ |
| SUN1mRFP1Flag | ND | ND | ND | + | +++ | +++ |
| Nesp3mRFP1 | ND | ND | ND | ND | ND | + |
| Nesp3mRFP1Nesp | ND | ND | ND | + | ++ | ++ |
| RANGAP1GFP | ND | ND | ND | ND | + | ++ |
ND not detected. Standard
aThe time (days) after induction to differentiate into MAs when the fluorescent protein was detected is indicated. Preadipocytes were nucleofected with five adiponectin promoted reporters, one for cytoplasmic expression (mRFP1) to test the vector pADNpcDNA3.1 KanR and four others targeting nuclei and designed to implement INTACT technology