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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biomaterials. 2016 Jun 2;101:272–284. doi: 10.1016/j.biomaterials.2016.05.051

Figure 2. CAT-NP activity within biofilms with pH dependent catalysis in situ.

Figure 2

a, Catalytic activity of CAT-NP adsorbed within biofilms. Inset: photographic images of CAT-NP treated biofilm before and after exposure to H2O2 and TMB (the blue color indicates free-radical generation via H2O2 catalysis in situ). b, Catalytic activity of CAT-NP (0.5 mg ml-1) treated biofilms at different pH. The difference of absorbance values between 2a and 2b is due to different incubation time used for each experiment. For figure 2a, we conducted the incubation with H2O2 and TMB for 30 min to allow all samples generate signals, particularly for those treated with low CAT-NP concentration. For Figure 2b (biofilms treated with 0.5 mg ml-1 CAT-NP), the activity was determined using the standard 5 min incubation with H2O2 and TMB.