Figure 2.

Simulated oral‐gastric digestion of wheat flour protein undertaken using a low pepsin protocol. Soluble (A, C) and insoluble (B, D) fractions of digests were analyzed by SDS‐PAGE (A, B) and immunoblotting (C, D) using mAb IFRN 0610 (C, D; brightened by + 40%). Selected bands (arrowed) were subjected to densitometric analysis (Supporting Information Table 3) and used for kinetic analysis. Soluble protein volumes loaded accounted for dilution factor between digestive phases whereas extracted insoluble protein samples were loaded in equivalent volumes. Pepsin was identified on the basis of its M_r defined by SDS PAGE analysis of the digestion enzymes (data not shown).