Table 2.
Nodule number, fresh weight, leghemoglobin content, nitrate and nitrite reductase, and nitrogenase activity of Acacia gerrardii nodules under salt stress after inoculation with B. subtilis and AMF alone and in combination.
| Treatments | NN | NFW | LG | NR | NiR | NG | CP | |
|---|---|---|---|---|---|---|---|---|
| 0 mM NaCl | Control | 57.1 ± 1.8 | 1.2 ± 0.04 | 6.2 ± 0.5 | 8.5 ± 0.7 | 6.1 ± 0.5 | 125.3 ± 10.3 | 593.1 ± 43.7 |
| BS | 92.9 ± 2.3 | 2.1 ± 0.07 | 8.9 ± 0.7 | 11.1 ± 0.9 | 8.1 ± 0.7 | 175.5 ± 12.6 | 887.9 ± 62.1 | |
| AMF | 71.9 ± 1.9 | 1.8 ± 0.05 | 7.4 ± 0.7 | 9.8 ± 0.8 | 6.7 ± 0.5 | 151.3 ± 11.2 | 729.7 ± 51.7 | |
| BS + AMF | 106.4 ± 2.7 | 2.4 ± 0.09 | 9.1 ± 0.8 | 12.1 ± 1.2 | 8.8 ± 0.8 | 185.7 ± 13.4 | 997.5 ± 83.6 | |
| 250 mM NaCl | Control | 10.9 ± 0.4 | 0.2 ± 0.01 | 1.2 ± 0.1 | 5.2 ± 0.3 | 3.2 ± 0.2 | 34.0 ± 3.1 | 163.8 ± 13.7 |
| BS | 45.9 ± 1.2 | 0.9 ± 0.02 | 4.7 ± 0.2 | 7.8 ± 0.5 | 4.3 ± 0.3 | 90.2 ± 8.4 | 493.5 ± 32.4 | |
| AMF | 31.4 ± 0.7 | 0.7 ± 0.01 | 3.1 ± 0.1 | 6.3 ± 0.4 | 4.1 ± 0.3 | 75.7 ± 6.4 | 408.1 ± 27.5 | |
| BS + AMF | 50.6 ± 1.6 | 1.0 ± 0.03 | 4.9 ± 0.3 | 8.1 ± 0.7 | 5.0 ± 0.4 | 98.7 ± 8.6 | 550.9 ± 37.8 | |
| LSD p < 0.05 | 2.75 | 0.07 | 0.44 | 0.19 | 0.59 | 2.00 | 14.97 | |
NN, number of nodules; LG, leghemoglobin (mg g−1 nodule FW); NR, nitrate reductase (μm NO2 released/h/g FW), NiR, nitrite reductase (μm NO2 disappeared/h/g FW); NG, nitrogenase (μmole ethylene/mg nodule FW/h); CP, crude protein (total nitrogen content as mg/g dry weight × 6.25); ±, standard deviation.