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. 2015 Oct 8;84(3):545–557. doi: 10.1111/tpj.13020

Figure 4.

Figure 4

taf1‐2 mutants express a mutant TAF1 transcript.

(a) taf1‐2 contains a SAIL T‐DNA inserted in the TAF1 5′ untranslated region. The TAF1 5′ UTR is shown in uppercase, introns as lower case and inserted sequence in italics. Analysis of left border sequences is consistent with a head‐to‐head double insertion of the T‐DNA.

(b) Comparison of TAF1 expression levels in Col‐0 and taf1‐2 mutants by semi‐quantitative PCR.

(c) Q‐PCR normalised to ACTIN2.

(d) Sequence analysis of the TAF1 transcript expressed in taf1‐2 mutants. ATG codons corresponding to translation AUG start sites in the messenger RNA are indicated in bold. Translation is shown for the ATG codons in frame with wild‐type TAF1 coding region. The position in corresponding to the SAIL T‐DNA Left Border primer is indicted by the box. An insertion at the T‐DNA/TAF1 border is shown in italics and homology with the T‐DNA is shown in bold. Splice sites are indicated by asterisks.