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. 2015 May 14;134(3):405–415. doi: 10.1111/jnc.13132

Figure 6.

Figure 6

Activity‐dependent bulk endocytosis (ADBE) is abolished by both BAPTA‐AM and EGTA‐AM. Cultures were preincubated with BAPTA‐AM, EGTA‐AM (both 100 μM) or DMSO (vehicle control) for 30 min before loading with tetramethylrhodamine‐dextran (TMR)‐dextran (50 μM) during stimulation with a train of 800 action potentials (80 Hz). (a) Quantification of the number of evoked TMR‐dextran puncta per field of view in cultures treated with either DMSO (Ctrl, blue bars) BAPTA‐AM (red bars) or EGTA‐AM (purple bars) ± SEM. ***p < 0.001, one‐way anova, n = 3 coverslips per condition. (b–f) DMSO, BAPTA‐AM or EGTA‐AM‐treated cultures were loaded with horse radish peroxidase (HRP) during stimulation with a train of 800 action potentials (80 Hz). (b) Representative images of nerve terminals after HRP loading in DMSO (Ctrl, B), BAPTA‐AM (c) or EGTA‐AM (d) ‐treated cultures. Scale bar indicates 100 nm. White arrow indicates HRP‐endosomes. Red arrow indicates HRP SVs. (e and f) Quantification of the number of HRP‐labelled endosomes (e) or HRP‐labelled SVs (f) ± SEM per nerve terminal in either Ctrl (blue bars), BAPTA‐AM (red bars) or EGTA‐AM (purple bars) –treated cultures. One‐way anova, ***p < 0.001, **p < 0.01, n = 3 independent experiments per condition.