Figure 1.
Three transglycanase activities of native Equisetum HTG.
(a–c) Three reactions catalysed by HTG, with labelled xyloglucan heptasaccharide (XXXGol) as acceptor substrate (for explanation of XGO nomenclature, see Fry et al., 1993). (d–f) Dot‐blot assays. Donor substrates: PP, plain paper (cellulose); AP, alkali‐washed paper; XP, xyloglucan‐impregnated paper; MP, MLG‐impregnated paper. Acceptor substrate: XXXG–sulforhodamine. Enzymes blotted comprise a dilution series (100, 50, 25, 12.5% v/v) of (NH 4)2 SO 4‐precipitated proteins from a grass (Holcus lanatus) or Equisetum (E. fluviatile and E. arvense). Polymeric reaction products were documented after sequential washing (d, e) and enzymolysis (f).