Figure 2.

Western blot analysis of purified His₆‐CotY crystal and supernatant fractions from E scherichia coli overexpression. Lane 1 contains untreated nickel‐affinity batch purified supernatant fraction. Lanes 2–5 show nickel‐affinity batch purified CotY crystals treated with combinations of denaturing conditions: heating at 99°C and 50 mM DTT over 20 min. Bands present at the top of lanes 2–5 indicate material trapped at the well interface. The recovered yield of crystals in the batch purification is not known, so protein signal in lane 1 cannot be compared with that in the ‘crystal’ lanes.