FOXP3 protein expression in the experimental and control groups was analyzed by western blot analysis. The results demonstrated high expression levels of FOXP3 in advanced gastric cancer. Lane 1, control group; lane 2, experimental group (grade I); lane 3, experimental group (grade II); lane 4, experimental group (grade III); lane 5, experimental group (grade IV). FOXP3, forkhead box protein 3.