Table 5.
Parameter set for simulating stopped‐flow traces with berkeley madonna, version 8.3.14. The reductive half‐reaction recorded for different electron donors (sugars) was simulated in agreement with Kinetic Model 1red. The oxidative half‐reaction for the electron acceptor BQ was simulated according to Kinetic Model 1ox. Simulated traces of the reductive and oxidative half‐reactions for AmPDH are shown in red dashed lines in Figs 6 and 7, respectively
| Variant | Substrate | k 1 (m −1·s−1) | k 2 (m −1·s−1) | εA (m −1·cm−1) | εB (m −1·cm−1) | εC (m −1·cm−1) |
|---|---|---|---|---|---|---|
| AmPDH | GLC | 6.9 × 104 | 5.4 | 9.0 × 103 | 1.1 × 103 | 0.7 × 103 |
| GAL | 5.2 × 104 | 4.0 | 9.3 × 103 | 1.0 × 103 | 0.8 × 103 | |
| ARA | 7.8 × 104 | 5.0 | 9.5 × 103 | 1.5 × 103 | 1.5 × 103 | |
| MGP | 6.5 × 104 | 1.0 | 9.3 × 103 | 1.5 × 103 | 1.3 × 103 | |
| BQ | 8.0 × 103 | 0.05 | 1.7 × 103 | 1.3 × 104 | 9.2 × 103 | |
| H556A | GLC | 1.0 × 102 | 8.0 × 101 | 1.3 × 104 | 9.8 × 103 | 1.1 × 103 |
| BQ | 3.6 × 104 | 1.6 × 103 | 1.2 × 103 | 2.0 × 104 | 1.1 × 104 |