Fig. 1.

MICAL1 regulates breast cancer migration and invasion in vitro. a MDA-MB-231 cells were transfected with negative control siRNA or siRNA specifically targeting MICAL1 (siMICAL1). 48 h later, total protein extracts from cells were analyzed by immunoblotting analysis for MICAL1 expression. *: P < 0.05 in the cells transfected with or without siRNA targeting MICAL1. (b&c) Representative transwell migration (b) and wound healing assay (c) images of control and MICAL1 silencing MDA-MB-231 cells. Quantification of migration rates was analyzed respectively. d MDA-MB-231 cells transfected with control siRNA or siMICAL1, and the quantification of cell invasion rate was performed. (e&f) MDA-MB-231 (e) and MCF-7 cells (f) were transfected with MICAL1 or empty vector, and the quantification of cell invasion rate was performed. *:P < 0.05, **:P < 0.01 in the cells transfected with HA–MICAL1 relative to cells transfected with the corresponding vector. g MCF-7 cells transfected with control siRNA or siMICAL1, and the quantification of cell invasion rate was performed. *: P < 0.05 in the siMICAL1 cells relative to siRNA control cells