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. 2016 Jul 18;16:489. doi: 10.1186/s12885-016-2553-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Active form of RAB35 binds to MICAL1. a Representative micrographs of MDA-MB-231 and MCF-7 cells stained for RAB35 (green) and MICAL1 expression (red) by immunofluorescence assay. Scale bar, 10 μm. b Coimmunoprecipitation experiments were performed with HEK293T cells cotransfected with HA-tagged MICAL1 and GFP-tagged RAB35. c MCF-7 cells were transfected with GFP-tagged RAB35 (WT), RAB35 (DN) or RAB35 (CA), and then immunoprecipitated with anti-GFP antibody, followed by immunoblotting analysis for RAB35 and MICAL1. d Binding of endogenous RAB35 to MICAL1 was detected in MDA-MB-231 cells and MCF-7 cells by coimmunoprecipitation experiments. n = 3 for all experiments