Fig. 3.

Effect of RAB35 on breast cancer cell invasion. a RAB35 and MICAL1 expressions were examined by immunoblotting in several types of malignant breast cancer cell line. b MDA-MB-231 cells were incubated with EGF (10 ng/mL) for indicated times, and analyzed for RAB35 activity by pulldown assays. *P < 0.05 in the cultures with EGF relative to the cultures without EGF. c MCF-7 cells were transfected with negative control siRNA or siRAB35. 48 h later, total protein extracts from cells were analyzed by immunoblotting analysis for RAB35 expression. Western blot bands corresponding to RAB35 were quantified and normalized against GAPDH level. **: P < 0.01 in the siRAB35 cells relative to control siRNA cells. d MCF-7 cells transfected with control siRNA or siRAB35, and the quantification of cell invasion rate was performed. *: P < 0.05 in the siMICAL1 cells relative to control siRNA cells. e Invasion assays results showed that knockdown of MICAL1 delayed cell invasion in RAB35 (CA)-expressing MDA-MB-231 cells. *:P < 0.05. **:P < 0.01