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. 2016 Jun 8;12(2):815–824. doi: 10.3892/ol.2016.4690

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Copyright: © Zeng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — DiI-retaining cells possess multipotency in vitro. (A) Expression of GFAP, βIII-tubulin and PDGFRα in DiI-retaining and DiI-negative cells in the NCH421k cell line prior to and following differentiation as assayed by western blot, with β-actin as the internal reference. Quantification of the band densities of (B-a) GFAP, (B-b) βIII-tubulin and (B-c) PDGFRα through normalization to β-actin using ImageJ software. The data are presented as the mean ± standard deviation from three independent experiments. *P<0.05 compared with expression prior to differentiation in the DiI-retaining group. GFAP, glial fibrillary acidic protein; PDGFRα, platelet-derived growth factor receptor α.