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. 2016 Jul 18;16:490. doi: 10.1186/s12885-016-2545-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — FPR1 stimulation promotes intracellular Ca2+ mobilization. a Changes in intracellular calcium level were measured by confocal scanning microscope using green fluorescent calcium-binding dye. After baseline measurement, fMLP was added to the cells’ media and subsequent fluctuations of green fluorescence were monitored. b Pretreatment with EDTA. The arrow depicts time of fMLP addition