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. 2016 May 26;12(2):695–701. doi: 10.3892/etm.2016.3397

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Albumin absorption aggravated neuronal damage in vitro. As compared with the (A) control group, (B) KA (10 uM) treatment in vitro induced significant neuronal damage. As compared with these groups, (C) KA + albumin (10 uM) and (D) KA + albumin (50 uM) treatment induced more severe neuronal damage and neuronal death in a dose- and time-dependent manner. Double staining of (E) NeuN and (F) albumin demonstrated a match between NeuN and albumin in vitro. (H) Cell counting showed reduced neuron number of albumin treatment in a dose- and time-dependent manner. *P<0.05, **P<0.01. Scale bar: (A-D), 100 µm; (E-G), 200 µm. KA, kainic acid.