Figure 3.

RPR extract induces cell cycle alterations. Cell cycle distribution was analyzed following RPR treatment. (A) Human bladder papillary transitional cell carcinoma BFTC 905 and (B) mice bladder carcinoma MB49 cells were treated with RPMI-1640 medium alone (0 mg/ml RPR) or RPR extract dissolved in RPMI-1640 medium (0.5, 1, 2, 3 and 3.5 mg/ml) for 24 h and 48 h, and subsequently collected for cell cycle analysis. Data are represented as the mean ± standard error of the mean. The experiment was repeated three times. *P<0.05; **P<0.01; ***P<0.001. RPR, Radix Paeoniae Rubra.