Figure 6.

Knockdown of MET reduced the cell viability and migration of lung adenocarcinoma cells. (A) A549, PC-9 and HCC827 cells were transfected with 40 nM NC or si-MET for 48 h. The cell lysates were harvested, and the phosphorylation of the indicated proteins was determined by western blotting. Three independent experiments were performed. (B) A549, PC-9 and HCC827 cells were transfected with 40 nM NC or si-MET for 48 h. Cell viability was determined using water-soluble tetrazolium-8 assays. Each experiment was performed three times in triplicate. The results are presented as the mean ± SD (*P<0.05, **P<0.01). (C) Quantification from three independent experiments and (D) representative micrographs of the cell migration assay (magnification, ×100). The results are presented as the mean ± SD (*P<0.05, **P<0.01). si, small interfering; NC, negative control; SD, standard deviation.