Figure 5.

Transport rates of [³H]glutamate and [³H]GABA in the presence of different external amino acids from transgenic tomato fruit tonoplast vesicles overexpressing SlCAT9‐YFP.

Tonoplast vesicles were isolated from mature green tomatoes of two independent transgenic lines (CAT 9_2 and CAT 9_5) and wild‐type (WT), pre‐loaded for 10 min with [³H]glutamate (a) or [³H]GABA, and the rate of efflux of [³H]glutamate (a) or [³H]GABA (b) measured in the presence of a range of amino acids (25 mm). A buffer control was included to test the zero‐trans configuration. Values are means ± standard error (SE) from three biological replicates consisting each of three technical replicates. *Indicates significantly different from WT (t‐test, P < 0.05).